1) correction of pH and dissolved oxygen electrodes.
2) tank sterilization. Medium into the tank as needed, as required when small fermentation tank (5L) tank can be placed in large autoclaves sterilizers (115 degrees, 30 minutes), large fermentation tanks (10L and above) by steam sterilization (121, 30min).
3) tank is cooled, place it in the fermentation stage, install good; open cooling water, power up the pump and start connecting ventilation pipe ventilation, adjust the air inlet knob ventilation suitable, keep the tank pressure in 0.05MPa; settings, such as temperature, pH, agitation speed, and in determining the speed and under the ventilation on the dissolved oxygen electrode slope calibration, value 100%
4) temperature stability parameters are correct, the rocking good seed access, start fermentation time, and began recording various parameters.
5) cleaning after completion of fermentation tank and electrodes, the pH electrode into the 3M flask for potassium chloride, dissolved oxygen electrode probes protective condom is good, save and set aside.